Environmental and cultivar variability in composition, content and biological activity of phenolic acids and alkylresorcinols of winter wheat grains from a multi-site field trial across Europe

Different factors such as the genotype, environmental conditions, temperature stress, solar radiation and others can influence phytochemical status of plants. The concentration phenolic acids alkylresorciols (ARs) well their chemical composition biological activity have been determined in twelve winter wheat cultivars grown at eight European locations. This was first multi-location field trial Consortium for Open Field Experimentation (ECOFE). Extracts from grain were analyzed using a UPLC-PDA-ESI-MS system (phenolic acids), UPLC-PDA-MS/MS (alkylresorcinols) TLC-DPPH• test with ImageJ program (antiradical activity). acid profile consisted five hydroxybenzoic four hydroxycinnamic derivatives, among which ferulic sinapic predominated. ARs nine AR 5-n-heneicosylresorcinol (C21:0) 5-n-nonadecanylresorcinol (C19:0) Our study showed significant differences content between cultivars, We observed positive correlation extracts total amount ARs. Two Chambo Julius (average all sites) samples Spanish site cultivars) highest nutritional substances.